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Bio-Scale DEAE Columns Instruction Manual
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website deae Cytiva HiTrap™ DEAE Sepharose Fast Flow IEX Columns Weak anion exchanger for small-scale protein purifications Cytiva Lifescience™ HiTrap™ DEAE Sepharose Fast deae As a starting point for developing a separation, we recommend using the Bio-Scale DEAE 2 column with a simple gradient profile over 48 ml Protocol: Use a flow
deae If the antibody solution is adjusted to a basic pH of 8–, then IgG binds to the DEAE resin After washing the column, the antibody is eluted by adding a The resin in G-Trap™ DEAE Agarose Fast Flow is a weak anion exchanger where diethylaminoethyl weak anion exchange group is coupled to agarose The Theoretically, HP-NAP is negatively charged above its pI However, the recombinant HP-NAP obtained from the unbound fractions from DEAE ion-exchange
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